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Erik Heyerdahl Strøm, MD
Bjørg Lund
Sverre-Henning Brorson, MSc,PhD
The Electron Microscopy Unit at the Dept of Pathology, Ullevål Hospital performs ultrastructural examination of tissue biopsies from patients located in the health region which is under the administration of Ullevål Hospital. The EM-laboratory has its main focus on renal biopsies. The renal biopsies are precisely examined by our specialist in renal pathology, Erik Heyerdahl Strøm. We have developed techniques which makes it possible to perform immunoelectron microscopy for immunoglobulin antigens (IgG, IgM, IgA) and complement on epoxy embedded renal biopsies (see the reference list). We also examines tumors of different kinds, immotile ciliary syndrome, and biopsies from a variety of tissues.
The laboratory is also focused on developing methods to improve the yield and quality of immunogold labeling of resin sections, especially epoxy sections (see the reference list). In the future we will also try to perform in situ hybridization at the ultrastructural level on resin section.
Lately, in the rooms of the EM-laboratory PCR-techniques are being developed.
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Scientific articles
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Here is a list of articles produced at our EM-unit over the last years:
1. The use of postembedding immunelectron microscopy in the diagnosis of glomerular diseases. Skjørten FJ, Brorson SH, Roald B, Strøm EH, Lund B: APMIS 100: 1001-1007, 1992
2. Neuronal uptake of plasma proteins in cryogenic brain lesions. Løberg EM, Brorson SH, Skjørten F and Torvik A: APMIS 100: 1033-1040, 1992
3. Antibody penetration into LR-White sections. Brorson SH, Roos N and Skjørten F. Micron 25: 453-460, 1994.
4. Mechanism for antigen detection on deplasticized epoxy sections. Brorson SH and Skjørten F. Micron 26: 301-310, 1995
5. The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections. Brorson SH and Skjørten F. Micron: 27: 193-201, 1996 Abstract
6. The theoretical ratio of immunogold labeling of deplasticized epoxy sections and acrylic sections. Brorson SH and Skjørten F. Micron: 27: 203-209, 1996
7. Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximately the same immunogold labeling for epoxy sections as for acrylic sections without any etching. Brorson SH and Skjørten F. Micron: 27: 211-217, 1996 Abstract
8. Improved immunogold labeling of epoxy sections by the use of propylene oxide as additional agent in dehydration, infiltration and embedding. Brorson SH. Micron: 27: 345-353, 1996
9. Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases. Brorson SH, Strøm EH, and Skjørten F. APMIS.: 105: 139-149, 1997
10. How to examine the antigen-damaging effect of sodium ethoxide on deplasticized epoxy sections. Brorson SH. J. Histochem. Cytochem. 45:143-146, 1997
11. Transformation of Cystic Forms of Borrelia burgdorferi to Normal Mobile Spirochetes. Brorson Ø, Brorson SH. Infection. 25: 240-246, 1997
12. Bovine Serum Albumin (BSA) as a Reagent against Non-specific Immunogold Labeling on LR-White and Epoxy Resin. Brorson SH. Micron. 28: 189-195, 1997
13. Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin. Brorson SH. Histol Histopathol. 13: 275-281, 1998
14. An easy method for generating cystic forms of Borrelia burgdorferi, and its use for characterizing the development from cystic forms to normal, mobile spirochetes. Brorson Ø, Brorson SH. AMPIS. In Press, 1998
15. In vitro conversion of Borrelia burgdorferi to cystic forms in spinal fluid, and transformation to mobile spirochetes by incubation in BSK-H medium. Brorson Ø, Brorson SH. Infection. 26: 144-150, 1998
16. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-White section for large proteins. Brorson SH. Micron. 29: 89-95, 1998 Abstract
17. Antigen retrieval on epoxy sections based on tissue infiltration with moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue. Brorson SH, Andersen T, Haug S, Kristiansen I, Risstubben A, Tchou H, Ulstein J. Histol Histopathol. In Press, 1999
18. Comparison of the immunogold labeling of single light chains and whole immunoglobulins with anti-k on LR-White sections and epoxy sections. Brorson SH. Micron. In press, 1998
19. A new immunoelectron microscopy approach for the detection of immunoglobulin and complement deposits in epoxy-embedded renal biopsies. Brorson SH, Strøm EH. Ultrastruct. Pathol. In press, 1999
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